A schematic overview of the sample processing and mePENK-LTE/qMSP assay for detecting bladder cancer (IMAGE)

Elsevier

A schematic overview of the sample processing and mePENK-LTE/qMSP assay for detecting bladder cancer

Caption

Fresh voided urine samples were collected into tubes containing preservative buffer. Total DNA was extracted from the urine sediments and underwent bisulfite conversion. Subsequently, linear target enrichment (LTE) and quantitative methylation-specific PCR (qMSP) were conducted to determine the level of PENK methylation associated with bladder cancer. A positive result indicates a higher probability of bladder cancer, suggesting further cystoscopy is needed to confirm the diagnosis. Conversely, a negative result indicates a lower or no risk of bladder cancer, thereby allowing for the avoidance or postponement of cystoscopy.

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Genomictree, Inc.

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