One-pot cell-free synthesis of mature iron–sulfur proteins (IMAGE)
Caption
This diagram shows the two main steps involved in the proposed synthesis protocol. Step 1 shows the chemical cascade used to create an oxygen-free environment, alongside the PURE system used to synthesise the 'immature' (apo) proteins. Step 2 shows the implementation of the SUF machinery, which adds the [4Fe–4S] cluster to the apo proteins, yielding functional and mature [4Fe–4S] proteins.
Credit
Reproduced from Wang and Nishikawa et al. 2023 ACS Synthetic Biology
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License
CC BY-NC-SA