Polarization-resolved SHG imaging of the cornea structure. (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Polarization-resolved SHG imaging of the cornea structure.

Caption

a, a simplified scheme of the polarization-resolved SHG microscope with epi-detection. The polarization is controlled by two waveplates at the back aperture of the objective lens. b, an example of a polarimetric diagram obtained in every voxel of the cornea. The double-arrow indicates the orientation of collagen in the imaging plane. c, Three-dimensional reconstruction of the lamellar structure of a human cornea. The colors indicate the direction of the collagen lamellae in the imaging plane, as indicated in the color wheel insert. Image size is 250 ×250×600 µm³. The anterior part of the cornea is at the top of the image.

Credit

by Marie-Claire SCHANNE-KLEIN

Usage Restrictions

Credit must be given to the creator.

License

CC BY

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